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East Tennessee State University

3 ARTICLES PUBLISHED IN JoVE

Biology

Using Caco-2 Cells to Study Lipid Transport by the Intestine

Andromeda M. Nauli¹, Judy D. Whittimore²

¹Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, ²Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University

Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.

Medicine

Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography

Stephanie L. C. Scofield¹, Krishna Singh^1,2

¹Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, ²James H. Quillen Veterans Affairs Medical Center

During murine myocardial ischemia/reperfusion surgery, correct placement of the occluding ligature is typically confirmed by visible observation of myocardial pallor. Herein, a method of electrocardiographically confirming ischemia and reperfusion, to supplement observed myocardial pallor, is demonstrated in male C57Bl/6 mice.

Bioengineering

Using Vertically Aligned Carbon Nanofiber Arrays on Rigid or Flexible Substrates for Delivery of Biomolecules and Dyes to Plants

Jessica M. Morgan¹, Joanna Jelenska², Dale K. Hensley³, Pengju Li⁴, Bernadeta R. Srijanto³, Scott T. Retterer^3,5, Robert F. Standaert⁶, Jennifer L. Morrell-Falvey⁵, Jean T. Greenberg²

¹Biophysical Sciences, The University of Chicago, ²Molecular Genetics and Cell Biology, The University of Chicago, ³Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, ⁴Pritzker School of Molecular Engineering, The University of Chicago, ⁵Biosciences Division, Oak Ridge National Laboratory, ⁶Department of Chemistry, East Tennessee State University

Here we describe methods for microfabricating vertically aligned carbon nanofibers (VACNFs), transferring VACNFs to flexible substrates, and applying VACNFs on both rigid and flexible substrates to plants for biomolecule and dye delivery.