The Chinese University of Hong Kong, Hong Kong Special Administrative Region

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

Wai Wa Ray Chan^*1, Wen Li^*1,2, Dik Long Dennis Chau^*1, Kwok-Fai Lau¹

¹School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, ²Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen

In this report, we describe a simple protocol for studying neurite outgrowth in embryonic rat cortical neurons by co-transfecting with EGFP and the protein of interest.

Biochemistry

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

Wai Wa Ray Chan^*1, Yu Qi Zhai^*1, Kwok-Fai Lau¹

¹School of Life Sciences, The Chinese University of Hong Kong

This paper aims to present a protocol for preparing recycling endosomes from mammalian cells using sucrose density gradient ultracentrifugation.