Mammalian Cell Culture, Transfection and Cell Harvest
2:07
Density Gradient Column Preparation
2:46
Fractionation and Recovery of Recycling Endosome-Containing Fraction
3:17
Results: Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
4:47
Conclusion
Transcript
This protocol helps in isolating the recycling endosomes containing fraction from transfected mammalian cells to facilitate the investigation of protein trafficking via endocytic trafficking. This technique is easy to handle and applicable to both
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This paper aims to present a protocol for preparing recycling endosomes from mammalian cells using sucrose density gradient ultracentrifugation.