Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction EnzymesApril Maschmann 1, Cody Masters 1, Melissa Davison 1, Joshua Lallman 1, Drew Thompson 1, Kristy L. Kounovsky-Shafer 1
1Department of Chemistry, University of Nebraska - Kearney
Staining DNA molecules for fluorescence microscopy allows a scientist to view them during an experiment. In the method presented here, DNA molecules are pre-stained with fluorescent dyes and digested with methylation and non-methylation sensitive restriction enzymes.
