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Yonsei University College of Medicine, Severance Hospital

1 ARTICLES PUBLISHED IN JoVE

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Biology

Thin Sectioning of Slice Preparations for Immunohistochemistry
Jae-Joon Park 1,2, Miles G. Cunningham 2
1Department of Medicine, Yonsei University College of Medicine, Severance Hospital, 2Department of Psychiatry, Harvard Medical School

The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.

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