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Thin Sectioning of Slice Preparations for Immunohistochemistry

DOI :

10.3791/194-v

April 28th, 2007

April 28th, 2007

32,372 Views

1Department of Medicine, Yonsei University College of Medicine, Severance Hospital, 2Department of Psychiatry, Harvard Medical School

The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.

Tags

Immunohistochemistry

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