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Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

large animal models

This collection features research methods using porcine, bovine, equine, and leporine (rabbit) models at their embryonic, neonatal, and adult stages. These techniques include ex-vivo and in-vivo studies, organ harvesting and preservation methods, cell isolation and culturing methodologies, microbial culturing, biochemical and biophysical assays, diagnostic tests, and various surgical procedures.

Large Animal Models

drosophila melanogaster (fruit fly)

This collection features research methods using the model organism Drosophila melanogaster at each stage of its life cycle to explore a wide range of physiological and behavioral questions.

Drosophila melanogaster (fruit fly)

caenorhabditis elegans (worm)

This collection features research techniques for the metazoan Caenorhabditis elegans. This nematode worm is a powerful model system due to its transparent body, defined developmental plan, robust genetic tools, and neuro-behavioral paradigms.

Caenorhabditis elegans (worm)

danio rerio (zebrafish)

This collection features research methods using the model organism Danio rerio in its embryo, larva, and adult stages to explore physiological and behavioral questions and create disease models for screening various chemicals.

Danio rerio (zebrafish)

rodent models

This collection features research methods using rodent models in embryonic, neonatal, and adult stages. The techniques include in vitro and in vivo organ perfusion, tissue and organ isolation, microbial culturing, biochemical studies, organ transplant methodologies, behavioral tests, and surgical procedures.

Rodent Models

embryo

Embryo

neonate

Neonate

adult

Adult

injection of microbubbles into isolated mouse embryos: a technique to deliver microbubble contrast agents into the vasculature of living murine embryos

injection-microbubbles-into-isolated-mouse-embryos-technique-to

Injection of Microbubbles into Isolated Mouse Embryos: A Technique to Deliver Microbubble Contrast Agents into the Vasculature of Living Murine Embryos

Source: Denbeigh, J. M. et al. Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound. J. Vis. Exp. (2015)
This video demonstrates a procedure ...

prenatal mouse embryos retrieval: a procedure to harvest embryos from pregnant mouse

prenatal-mouse-embryos-retrieval-procedure-to-harvest-embryos-from

Prenatal Mouse Embryos Retrieval: A Procedure to Harvest Embryos from Pregnant Mouse

Source: Yun, K. Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection. J. Vis. Exp.&#160;(2017)
This video demonstrates the ...

methylene blue dye injection in mouse embryonic urinary tract: a method to assess the congenital obstruction in the urinary tract

methylene-blue-dye-injection-mouse-embryonic-urinary-tract-method-to

Methylene Blue Dye Injection in Mouse Embryonic Urinary Tract: A Method To Assess the Congenital Obstruction in the Urinary Tract

Source: Yun, K. Assessing Urinary Tract Junction Obstruction Defects by Methylene Blue Dye Injection. J. Vis. Exp. (2017)
In this video, we administer ...

modeling transuterine fetal tracheal occlusion in murine model: a surgical procedure for ligation of the fetal trachea within a pregnant mouse

modeling-transuterine-fetal-tracheal-occlusion-murine-model-surgical

Modeling Transuterine Fetal Tracheal Occlusion in Murine Model: A Surgical Procedure for Ligation of the Fetal Trachea Within a Pregnant Mouse

Source: Ayd&#305;n, E. et al. Transuterine Fetal Tracheal Occlusion Model in Mice. J. Vis. Exp. (2021)
This video demonstrates the transuterine tracheal ...

neonatal mouse ovary isolation: a surgical procedure to harvest pair of ovaries from neonatal mouse model

neonatal-mouse-ovary-isolation-surgical-procedure-to-harvest-pair

Neonatal Mouse Ovary Isolation: A Surgical Procedure to Harvest Pair of Ovaries from Neonatal Mouse Model

Source: et al. Morgan, S. Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles. J. Vis. Exp. (2015).
In this video, we describe a surgical ...

white and brown adipose depot collection from mouse pup: a surgical procedure to harvest the white adipose tissue and brown adipose tissue from mouse pup

white-brown-adipose-depot-collection-from-mouse-pup-surgical

White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup

Source: Galmozzi, A. et al. Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice. J. Vis. Exp. (2021)
In this video, ...

apical resection neonatal mouse model: a surgical procedure to amputate the ventricular apex to study regenerative potential in a neonate mouse heart

apical-resection-neonatal-mouse-model-surgical-procedure-to-amputate

Apical Resection Neonatal Mouse Model: A Surgical Procedure to Amputate the Ventricular Apex to Study Regenerative Potential in a Neonate Mouse Heart

Source: Li, Y. et al. Transplantation of Neonatal Mouse Cardiac Macrophages into Adult Mice. J. Vis. Exp. (2021).
In this video, we demonstrate the ...

dissection of palate tissue from adult murine model: a surgical procedure to harvest hard palate and soft palate from oral cavity of adult mouse

dissection-palate-tissue-from-adult-murine-model-surgical-procedure

Dissection of Palate Tissue from Adult Murine Model: A Surgical Procedure to Harvest Hard Palate and Soft Palate from Oral Cavity of Adult Mouse

Source: Xuan Ngo, Y. et al. Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate.&#160;J. Vis. Exp.&#160;(2021)
This video ...

isolation of circumvallate papillae (cvp) epithelium from mouse model: an enzymatic procedure to separate cvp epithelium from mouse tongue

isolation-circumvallate-papillae-cvp-epithelium-from-mouse-model-an

Isolation of Circumvallate Papillae (CVP) Epithelium from Mouse Model: An Enzymatic Procedure to Separate CVP Epithelium from Mouse Tongue

Source: Shechtman, L. A. et al. Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells. J. Vis. Exp. (2021)
In this video, ...

mouse antral oocyte isolation: a method to isolate antral oocytes from freshly harvested mouse ovaries

mouse-antral-oocyte-isolation-method-to-isolate-antral-oocytes-from

Mouse Antral Oocyte Isolation: A Method to Isolate Antral Oocytes from Freshly Harvested Mouse Ovaries

Source: Monti, M. et al. Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization. J. Vis. Exp. (2016)
This video ...

exertional heat stroke mouse model: a protocol to study mechanisms underlying exertional heat stroke

exertional-heat-stroke-mouse-model-protocol-to-study-mechanisms

Exertional Heat Stroke Mouse Model: A Protocol to Study Mechanisms Underlying Exertional Heat Stroke

Source: King, M. A. et al. A Preclinical Model of Exertional Heat Stroke in Mice. J. Vis. Exp. (2021).
In this video we describe a step-by-step protocol ...

high-frequency ultrasonography based early pregnancy characterization: a technique to study embryo implantation and pregnancy progression in pregnant murine model

high-frequency-ultrasonography-based-early-pregnancy-characterization

High-frequency Ultrasonography Based Early Pregnancy Characterization: A Technique to Study Embryo Implantation and Pregnancy Progression in Pregnant Murine Model

Source: Peavey, M. C. et al. A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse. J. Vis. ...

cardiopulmonary complex decellularization: a technique for decellularizing heart and lungs from murine model

cardiopulmonary-complex-decellularization-technique-for

Cardiopulmonary Complex Decellularization: A Technique for Decellularizing Heart and Lungs from Murine Model

Source: Mayorca-Guiliani, A. E. et al. Decellularization of the Murine Cardiopulmonary Complex. J. Vis. Exp. (2021)
This video demonstrates a method of ...

generating murine model of myocardial infarction: a surgical procedure for permanent ligation of left anterior descending coronary artery in mouse model

generating-murine-model-myocardial-infarction-surgical-procedure-for

Generating Murine Model of Myocardial Infarction: A Surgical Procedure for Permanent Ligation of Left Anterior Descending Coronary Artery in Mouse Model

Source: Lugrin, J. et al. Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery. J. Vis. Exp. (2019)
In ...

swab-based conjunctival commensal bacteria isolation: a technique to isolate commensal bacteria from conjunctiva of murine model

swab-based-conjunctival-commensal-bacteria-isolation-technique-to

Swab-based Conjunctival Commensal Bacteria Isolation: A Technique to Isolate Commensal Bacteria from Conjunctiva of Murine Model

Source: Smith-Page, K. et al. Conjunctival Commensal Isolation and Identification in Mice. J. Vis. Exp. (2021)
This video describes a swab-based technique ...

surgical implantation of temperature telemetric monitoring system in mouse model: a surgical procedure to implant temperature telemetric device to measure mouse core body temperature

surgical-implantation-temperature-telemetric-monitoring-system-mouse

Surgical Implantation of Temperature Telemetric Monitoring System in Mouse Model: A Surgical Procedure to Implant Temperature Telemetric Device to Measure Mouse Core Body Temperature

Source: King, M. A. et al.&#160; A Preclinical Model of Exertional Heat Stroke in Mice. J. Vis. Exp. (2021)
In this video, we describe a surgical ...

imaging fixture supported mouse liver staging for multiphoton microscopy based vascular visualization: a stabilization device-assisted imaging technique to obtain multi-dimensional images of blood vessels in mouse liver

imaging-fixture-supported-mouse-liver-staging-for-multiphoton

Imaging Fixture Supported Mouse Liver Staging for Multiphoton Microscopy Based Vascular Visualization: A Stabilization Device-assisted Imaging Technique to Obtain Multi-dimensional Images of Blood Vessels in Mouse Liver

Source: Rongrong, W. et al. Multiphoton Microscopic Observation of Vessels in Mouse Liver Tissue. J. Vis. Exp. (2021)
This video describes an imaging ...

langendorff retrograde heart perfusion: an ex vivo technique to perfuse isolated mouse heart

langendorff-retrograde-heart-perfusion-an-ex-vivo-technique-to

Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart

Source: Wu, K. et al. Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice. J. Vis. Exp. ...

acute biliary pancreatitis model: a method to generate acute biliary pancreatitis mouse model via pancreatic duct infusion

acute-biliary-pancreatitis-model-method-to-generate-acute-biliary

Acute Biliary Pancreatitis Model: A Method to Generate Acute Biliary Pancreatitis Mouse Model via Pancreatic Duct Infusion

Source: Serra, M. B. et al. Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice. J. Vis. Exp. (2021)
This video describes the procedure ...

isolation of mouse valve interstitial cells: an enzymatic method to isolate and culture vics from mouse aortic valve

isolation-mouse-valve-interstitial-cells-an-enzymatic-method-to

Isolation of Mouse Valve Interstitial Cells: An Enzymatic Method to Isolate and Culture VICs from Mouse Aortic Valve

Source: Bouchareb, R. et al. Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve&#160;In Vitro.&#160;J. Vis. Exp. ...

transverse aortic arch banding in mouse model: a minimally invasive technique in mice for induction of high pressure in the left ventricle

transverse-aortic-arch-banding-mouse-model-minimally-invasive

Transverse Aortic Arch Banding in Mouse Model: A Minimally Invasive Technique in Mice for Induction of High Pressure in the Left Ventricle

Source: Zaw, A. M. et al. Minimally Invasive Transverse Aortic Constriction in Mice. J. Vis. Exp. (2017)
In this video, we demonstrate an experimental ...

surgical aortic debanding: a procedure to study left ventricular reverse remodeling in murine model of aortic constriction

surgical-aortic-debanding-procedure-to-study-left-ventricular-reverse

Surgical Aortic Debanding: A Procedure to Study Left Ventricular Reverse Remodeling in Murine Model of Aortic Constriction

Source: Goncalves-Rodrigues, P. et al. Studying Left Ventricular Reverse Remodeling by Aortic Debanding in Rodents. J. Vis. Exp. (2021)
This video ...

oral glucose tolerance test (ogtt): measuring glucose metabolism in mice

oral-glucose-tolerance-test-ogtt-measuring-glucose-metabolism-in-mice

Oral Glucose Tolerance Test (OGTT): Measuring Glucose Metabolism in Mice

Source: Ajoy, R. and Chou, S. Y. Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into ...

subcutaneous dihydrotestosterone (dht) treatment: mouse model of polycystic ovary syndrome (pcos)

subcutaneous-dihydrotestosterone-dht-treatment-mouse-model-polycystic

Subcutaneous Dihydrotestosterone (DHT) Treatment: Mouse Model of Polycystic Ovary Syndrome (PCOS)

Source: Xue, P., et al. A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome.&#160;J. Vis. Exp. (2018).
This article describes a way to ...

barnes maze: a method for assessing spacial learning and memory in lab animals

barnes-maze-method-for-assessing-spacial-learning-memory-lab

Barnes Maze: A Method For Assessing Spacial Learning and Memory in Lab Animals

Source: Ingersoll, J. et al. Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms. J. Vis. ...

assessing gait by footprint analysis: a method to test motor coordination in mice

assessing-gait-footprint-analysis-method-to-test-motor-coordination

Assessing Gait by Footprint Analysis: A Method to Test Motor Coordination in Mice

Source: Sugimoto, H. and Kawakami, K. Low-cost Protocol of Footprint Analysis and Hanging Box Test for Mice Applied the Chronic Restraint Stress.&#160;J. ...

systemic pilocarpine treatment: mouse model of temporal lobe epilepsy

systemic-pilocarpine-treatment-mouse-model-of-temporal-lobe-epilepsy

Systemic Pilocarpine Treatment: Mouse Model of Temporal Lobe Epilepsy

Source: Kim, J. E. and Cho, K. O. The Pilocarpine Model of Temporal Lobe Epilepsy and EEG Monitoring Using Radiotelemetry System in Mice. J. Vis. Exp. ...

the accelerating rotating rod assay or rotarod test: a method to test motor coordination and learning in mice

the-accelerating-rotating-rod-assay-or-rotarod-test-method-to-test

The Accelerating Rotating Rod Assay or Rotarod Test: A Method to Test Motor Coordination and Learning in Mice

Source: Ferro, A., et al. Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function.&#160;J. Vis. Exp.&#160; ...

the water maze with platform relocation: a method to assess working memory

the-water-maze-with-platform-relocation-method-to-assess-working

The Water Maze with Platform Relocation: A Method to Assess Working Memory

Source: Hausser, N., et al. Detecting Behavioral Deficits in Rats After Traumatic Brain Injury.&#160;J. Vis. Exp (2018).
In this video, we describe the ...

open field behavior test: a method to assess general locomotion and exploration habits

open-field-behavior-test-method-to-assess-general-locomotion

Open Field Behavior Test: A Method to Assess General Locomotion and Exploration Habits

Source: Osmon, K. J., et al. Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice. J. Vis. Exp. (2018).
...

in vivo murine liver perfusion: a method to infuse the rodent liver with solution via the portal vein

in-vivo-murine-liver-perfusion-method-to-infuse-rodent-liver-with

In Vivo Murine Liver Perfusion: A Method to Infuse the Rodent Liver with Solution via the Portal Vein

Source: Cabral, F., et al. Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. J. Vis. Exp. (2018).
This video ...

mouse headpost implantation: a method for head fixation in rodents

mouse-headpost-implantation-a-method-for-head-fixation-in-rodents

Mouse Headpost Implantation: A Method for Head Fixation in Rodents

Source: Fran&#231;a de Barros, F., et al. Long-term Sensory Conflict in Freely Behaving Mice. J. Vis. Exp. (2019).
This video shows a headpost ...

mouse echocardiography: a non-invasive technique to assess cardiac morphology

mouse-echocardiography-non-invasive-technique-to-assess-cardiac

Mouse Echocardiography: A Non-invasive Technique to Assess Cardiac Morphology

Source: Baudouy, D., et al. Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse. J. Vis. Exp. (2017).
This video describes a ...

filament insertion-based transient stroke model: a surgical procedure to perform middle cerebral artery occlusion in murine brain

filament-insertion-based-transient-stroke-model-surgical-procedure-to

Filament Insertion-based Transient Stroke Model: A Surgical Procedure to Perform Middle Cerebral Artery Occlusion in Murine Brain

Source: Llovera, G., et al. Modeling Stroke in Mice: Transient Middle Cerebral Artery Occlusion via the External Carotid Artery. J. Vis. Exp (2021).
This ...

biliary system resin casting: a technique for analyzing 3d architecture of biliary system in murine model using radiopaque resin injection

biliary-system-resin-casting-technique-for-analyzing-3d-architecture

Biliary System Resin Casting: A Technique for Analyzing 3D Architecture of Biliary System in Murine Model Using Radiopaque Resin Injection

Source: Hankeova, S. et al. DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis. J. Vis. Exp. (2021)
This video ...

modeling photothrombotic stroke in mouse model: a laser illumination technique through mouse skull following photosensitive dye administration to induce photothrombosis

modeling-photothrombotic-stroke-mouse-model-laser-illumination

Modeling Photothrombotic Stroke in Mouse Model: A Laser Illumination Technique Through Mouse Skull Following Photosensitive Dye Administration to Induce Photothrombosis

Source: Llovera, G. et al. Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis. J. Vis. Exp. (2021)
This video demonstrates the procedure ...

murine model of islet transplantation: a surgical method to infuse pancreatic islets in portal vein and prevention of postoperative bleeding

murine-model-islet-transplantation-surgical-method-to-infuse

Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding

Source: Gou, W. et al. Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice. J. Vis. Exp. (2021)
In this video, ...

sinoatrial node isolation from mouse model: a procedure to harvest sa node from murine heart

sinoatrial-node-isolation-from-mouse-model-procedure-to-harvest-sa

Sinoatrial Node Isolation from Mouse Model: A Procedure to Harvest SA Node from Murine Heart

Source: Kumar, P et al., Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice. J. Vis. ...

intact ovary harvest from mouse: a surgical procedure to isolate complete ovary from murine model

intact-ovary-harvest-from-mouse-surgical-procedure-to-isolate

Intact Ovary Harvest from Mouse: A Surgical Procedure to Isolate Complete Ovary from Murine Model

Source: Mason, J. B. et al. Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in ...

cranial ring assembly installation in mouse model: a surgical procedure to implant visualization window assembly on murine skull for high-resolution skull bone marrow imaging

cranial-ring-assembly-installation-mouse-model-surgical-procedure-to

Cranial Ring Assembly Installation in Mouse Model: A Surgical Procedure to Implant Visualization Window Assembly on Murine Skull for High-resolution Skull Bone Marrow Imaging

Source: Bornert, A. et. al., In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow. J. Vis. Exp. (2021)
In this ...

pancreatic intravital imaging window installation in mouse model: an integrated pancreatic imaging window for time-lapse in vivo imaging of murine pancreas microstructure

pancreatic-intravital-imaging-window-installation-mouse-model-an

Pancreatic Intravital Imaging Window Installation in Mouse Model: An Integrated Pancreatic Imaging Window for Time-lapse In Vivo Imaging of Murine Pancreas Microstructure

Source: Park, I. et. al. Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital ...

intravital imaging: a method for cellular level observation of the mouse pancreas through a stabilized imaging window

intravital-imaging-method-for-cellular-level-observation-mouse

Intravital Imaging: A Method for Cellular Level Observation of the Mouse Pancreas Through a Stabilized Imaging Window

Source: Park, I. and Kim, P. Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital ...

Encyclopedia Of Experiments - biology