Erratum: A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Resumen

An erratum was issued for: A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro. The Protocol section was updated.

Resumen

This corrects the article 10.3791/64016

Protocolo

An erratum was issued for: A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro. The Protocol section were updated.

Step 1.1 of the Protocol has been updated from:

Preparation of calcium stock solution (25 mM CaCl₂·2H₂O, 1.37 mM NaCl, 15 mM MgCl₂·6H₂O in Tris buffer)

to:

Preparation of calcium stock solution (25 mM CaCl₂·2H₂O, 1.37 M NaCl, 15 mM MgCl₂·6H₂O in Tris buffer)

Step 1.1.1 of the Protocol has been updated from:

Prepare 1.0 M Tris buffer and adjust pH to 7.4 using 1 M HCl.

to:

Prepare 50 mM Tris buffer and adjust pH to 7.4 using 1 M HCl.

Step 1.1.2 of the Protocol has been updated from:

Set up a glass beaker on a magnetic stirrer and add 100 mL of 1.0 M Tris buffer.

to:

Set up a glass beaker on a magnetic stirrer and add 100 mL of 50 mM Tris buffer.

Step 1.2 of the Protocol has been updated from:

Preparation of phosphate stock solution (11.1 mM Na2HPO4·H2O, 42 mM NaHCO3 in Tris buffer)

to:

Preparation of phosphate stock solution (11.1 mM Na2HPO4, 42 mM NaHCO3 in Tris buffer)

Step 1.2.1 of the Protocol has been updated from:

Set up a glass beaker on a magnetic stirrer and add 100 mL of 1.0 M Tris buffer.

to:

Set up a glass beaker on a magnetic stirrer and add 100 mL of 50 mM Tris buffer.

Step 1.2.2 of the Protocol has been updated from:

Weigh out 0.158 g of Na2HPO4·H2O and 0.353 g of NaHCO3 and dissolve in Tris buffer one by one.

to:

Weigh out 0.158 g of Na2HPO4 and 0.353 g of NaHCO3 and dissolve in Tris buffer one by one.

Step 1.3.1 of the Protocol has been updated from:

Prepare 30 mL of working solution by mixing 15 mL of 1.0 M Tris buffer, 7.5 mL of calcium stock (step 1.1.4) solution, and 7.5 mL of phosphate stock solution (step 1.2.3). Filter the solution with a 0.2 µm filter.

to:

Prepare 30 mL of working solution by mixing 15 mL of 50 mM Tris buffer, 7.5 mL of calcium stock (step 1.1.4) solution, and 7.5 mL of phosphate stock solution (step 1.2.3). Filter the solution with a 0.2 µm filter.

Step 1.4 of the Protocol has been updated from:

Preparation of calcium phosphate solution (2.25 mM Na2HPO4·H2O, 4 mM CaCl2·2H2O, 0.14 M NaCl, 50 mM Tris base in ddH2O)

to:

Preparation of calcium phosphate solution (2.25 mM Na2HPO4, 4 mM CaCl2·2H2O, 0.14 M NaCl, 50 mM Tris base in ddH2O)

Step 1.4.1 of the Protocol has been updated from:

Add 2 mL of 1 M HCl to 40 mL of deionized water in a beaker with a magnetic stirring bead and dissolve 0.016 g of Na2HPO4·H2O, 0.0295 g of CaCl2·2H2O, 0.409 g of NaCl, and 0.303 g of Tris base one by one.

to:

Add 2 mL of 1 M HCl to 40 mL of deionized water in a beaker with a magnetic stirring bead and dissolve 0.016 g of Na2HPO4, 0.0295 g of CaCl2·2H2O, 0.409 g of NaCl, and 0.303 g of Tris base one by one.