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13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement (Video) | JoVE

DOI :

10.3791/200017-v

•

4:55 min

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May 19th, 2023

May 19th, 2023

•
179 Views
1Program in Molecular Medicine, University of Massachusetts Chan Medical School, 2Cancer Center, University of Massachusetts Chan Medical School
* These authors contributed equally

Tags

13C4 Aspartate
13C5 Glutamine
Stable Isotope Tracing
LC MS Analysis
SDH Activity Measurement
Seed Cells
Six well Plate
Medium Replacement
Steady State Labeling
Metabolite Isolation
Buffer Preparation
Cell Lysis
Incubation
Scraping Cells
Cell Lifter
Microcentrifuge Tube
Vortexing Lysate
Centrifugation
Supernatant Transfer
Vacuum Concentration
Sample Analysis
Liquid Chromatography Mass Spectrometry
LCMS

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13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

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