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Transfection and Transduction of Murine T-Cells for CAR-T Cell Generation

335 Views

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05:34 min

•

February 2nd, 2024

DOI :

10.3791/200881-v

February 2nd, 2024

•
335 Views
1Department of Biomedical Engineering, Columbia University, 2Department of Microbiology & Immunology, Vagelos College of Physicians and Surgeons, Columbia University, 3Herbert Irving Comprehensive Cancer Center, Columbia University, 4Data Science Institute, Columbia University

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Keywords Murine T cells
CAR T Cell Generation
Transfection
Transduction
Phoenix Eco Plate
Viral Supernatant
Splenocytes
CD3 Positive T cells
Negative Selection
T cell Activation
Human Fibronectin Transduction Enhancer

De la serie

Streamlined Protocol for Mouse CAR-T Cell Generation in Syngeneic Models
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