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Absorbance-Based Microplate Analysis to Test the Serum Stability of Human Adenosine Deaminase 1

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15 Views

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02:28 min

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September 27th, 2024

DOI :

10.3791/202214-v

September 27th, 2024

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Yusef Haikal¹, Maria Rain Jennings², Johnathan Nguyen¹, John J. Blazeck¹

¹School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, ²Johns Hopkins University School of Medicine

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Absorbance based Analysis

Microplate Reader

Serum Stability

Human Adenosine Deaminase 1

Adenosine Stock Preparation

Diluted Protein Samples

Absorbance Measurement

Slope Determination

Fraction Of Activity Remaining

Wildtype HsADA1 Activity

Absorbance Decline Curves

Negative Control Samples

De la serie

Evaluating Serum Stability of HsADA1: A Microplate-Based Approach

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