preparation of mice and carrageenan solution for creating a model of inflammatory pain

<meta charset="utf8"></head><body>Inyección doble en la pata trasera para mejorar las pruebas analgésicas

Pain is a public health problem worldwide. It is triggered by noxious thermal, mechanical, or chemical stimulation. It is difficult to perform pain studies in humans because of ethical concerns. Therefore, using animal pain models is a key approach to understanding pain mechanisms and research treatments1. The hot plate test has been widely used for the assessment of thermal nociception and hyperalgesia2. Currently, the model of inflammatory pain used for the hot plate test is induced with an intraplantar injection of carrageenan in one hind paw. For example, to investigate the antinociceptive effect of rotigotine-loaded microspheres, an animal model of inflammatory pain was prepared by a single-hind-paw injection3. To assess the effects of catechol-o-methyl-transferase inhibitors on thermal nociception in mice, carrageenan was administered into the plantar region of the right hind paw4. To investigate the analgesic role of the extract of Posidonia oceanica (L.) Delile, an inflammatory pain model was prepared in mice by intraplantar injection of carrageenan in the right hind paw5. However, the findings from our laboratory showed that a mouse with the single-hind-paw injection of carrageenan would lift its paw to avoid thermal nociception, which inaccurately extends the animal's response to a thermal stimulus in the hot plate test. Thus, we hypothesized that the inflammatory pain model with the single-hind-paw injection of carrageenan could not accurately reflect the thermal pain threshold. Therefore, we optimized the method by injecting carrageenan into both hind paws to establish a modified inflammatory pain model. 
The mice subjected to both-hind-paw injection of carrageenan had no way to avoid the thermal stimulus for both hind paws at the same time. The pain threshold was defined as the time from placing the mouse on the hot plate to the mouse licking either hind paw. The results confirmed that the modified inflammatory pain model is more suitable for evaluating the nociceptive temperature threshold. This may improve the efficacy of the hot plate test for the research and development of analgesic drugs.

<meta charset="utf8"></head><body>Autor destacado: Método mejorado para evaluar los efectos analgésicos: inyección doble de carragenina en la pata trasera en ratones

Un modelo de dolor inflamatorio modificado para estudiar el efecto analgésico en ratones

Pain has a negative impact on quality of life. The method for evaluating pain plays a key role in developing analgesic drugs. The present study tried to provide a reliable and superior method to evaluate analgesic effects.The hot plate test is widely used to evaluate analgesic effects on inflammatory pain in mice. A commonly used method of inflammatory pain was induced with an intraplantar injection of carrageenin in one hind paw. We found that mice with a single hind paw carrageenin injection lifted their paws to avoid the during the hot plate test.Because of this response, the injection method cannot accurately the mouse's pain threshold. Thus, we modified the previous method by injecting carrageenin into both hind paws to establish the model of inflammatory pain. The results demonstrated that both hind paw injection with carrageenin are sensitive, and the better method to induce inflammatory pain when used in the hot plate test than single hind paw injection.In summary, we developed a superior method to induce a model inflammatory pain to evaluate analgesic effects. The present experiment used only ICR mice. To test the purposes in the future study, we will investigate whether this modified method accurately and consequently detects the pain associated in the other mouse's stress.

Preparación de ratones y carragenina solución para crear un modelo de dolor inflamatorio

preparation-mice-carrageenan-solution-for-creating-model-inflammatory

Research

JoVE Journal

Neuroscience

Preparation of Mice and Carrageenan Solution for Creating a Model of Inflammatory Pain

143 vistas. Comience usando ratones hembra ICR que pesen de 20 a 25 gramos para los experimentos. Permita que los animales se aclimaten al entorno de la sala de experimentación durante tres días antes de comenzar los experimentos. Para preparar la solución de carragenina, pese 0,1 gramos de carragenina, agregue 10 mililitros de solución salina normal esterilizada a un tubo, agregue 0,1 gramos de carragenina al tubo y agite el tubo durante 30 segundos.A continu...

Video: Preparación de ratones y carragenina solución para crear un modelo de dolor inflamatorio

A Modified Inflammatory Pain Model to Study the Analgesic Effect in Mice

The hot plate test is widely used to evaluate analgesic effects on inflammatory pain in mice. A commonly used model of inflammatory pain was induced with an intraplantar injection of carrageenan in one hind paw. However, the findings from our laboratory showed that mice with a single-hind-paw injection of carrageenan lifted their paws to avoid thermal nociception during the hot plate test. Because of this response,&#160;previous injection method cannot accurately reflect the thermal pain threshold. Thus, we investigated a new method to avoid this issue. In the present study, we modified the previous method by injecting carrageenan into both hind paws to establish the model of inflammatory pain. The results demonstrated that both-hind-paw injection with carrageenan was sensitive and a better method to induce inflammatory pain when using the hot plate test than single-hind-paw injection. On the basis of these findings, we designed further experiments in which mice with either both-hind-paw or single-hind-paw injection of carrageenan were treated intragastrically with celecoxib (30 mg/kg). The results of the hot plate test showed that celecoxib augmented the thermal pain threshold in mice with both-hind-paw injection of carrageenan but not in mice with single-hind-paw injection of carrageenan. In summary, we developed a superior method to induce a model of inflammatory pain to evaluate analgesic effect.

Here, we present a modified inflammatory pain model with the both-hind-paw carrageenan injection to evaluate the analgesic effect.

The hot plate test is widely used to evaluate analgesic effects on inflammatory pain in mice. A commonly used model of inflammatory pain was induced with ...

Investigación

Neurociencias

Begin by using ICR female mice weighing 20 to 25 grams for the experiments. Allow the animals to acclimate to the experimental room environment for three days before starting the experiments. To prepare carrageenan solution, weigh 0.1 grams of carrageenan, add 10 milliliters of sterilized normal saline to a tube, add 0.1 grams of carrageenan to the tube, and vortex the tube for 30 seconds.Then collect the 1%sterilized carrageenan solution.

Establishing a Modified Inflammatory Pain Model with Both-Hind-Paw Carrageenan Injection

To begin, take a syringe to inject the prepared 1%Carrageenan solution. Shake the Carrageenan solution and draw it into the syringe while ensuring that there is no bubble in the syringe. Then, disinfect the injection site with 75%alcohol.Insert the needle diagonally upwards into the hind paw foot pad while maintaining the stability of the syringe to inject 30 microliters of 1%Carrageenan solution. After injection, withdraw the syringe slowly and press on the injection site for two seconds to prevent leakage. Clean and disinfect the surface of the hot plate.Start the hot plate apparatus and click the Temperature Mode button. Then, click Constant to set the temperature to approximately 55 degrees Celsius. Click Start Experiment button and click Reach.Now, take a mouse from the accommodation container. Gently grasp its trunk and place it in the center of the hot plate. Then, click Start.Observe and record the time to lift the left hind paw or hind paw in response to the heat stimulation. Press the pedal of the hot plate to see the time on the hot plate screen. Then set a 60 second limit for the hot plate test.Record a pain threshold of 60 seconds if the limit is reached. Take one capsule, containing 200 milligrams of Celecoxib. Get the content of the capsule and grind it into a powder.To prepare the store suspension of Celecoxib, add 0.5%sodium carboxy methylcellulose to make a final volume of 13.3 milliliters. Then take one milliliter of the store suspension of Celecoxib and add 9.0 milliliters of 0.5%sodium carboxy methylcellulose to obtain Celecoxib suspension. Administer 0.2 milliliters per 10 grams of the Celecoxib suspension, intragastrically to the mice.Randomly divide ICR female mice into three groups, control, model and Celecoxib groups. Inject 30 microliters of 1%Carrageenan solution subcutaneously into the planter surface of the hind paw. After the injection, intragastrically administer Celecoxib at 30 milligrams per kilogram for the mice in the Celecoxib group.Conduct the hot plate test at three hours after Carrageenan injection. The pain threshold of the single hind paw injection model did not show a significant reduction, compared with the control group. The pain threshold of the both hind paw injection model decreased significantly compared with the control group.In the single hind paw injection model, the pain threshold of the Celecoxib group did not significantly increase, compared to the model group. However, in the both hind paw injection model, the pain threshold of the Celecoxib group showed a significant increase.