Iniciar sesión

Se requiere una suscripción a JoVE para ver este contenido.

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

11.8K Views

08:10 min

May 29th, 2012

May 29th, 2012

11,791 Views

0:05

Title

2:27

Preparation of the Magnetic Beads

3:00

Affinity Capture Assay

4:18

Recycling the Beads

5:44

Interpretation of the Results

6:13

Results: Liquid Phase Affinity Capture Assay using PVSS38C Nanobody

7:36

Conclusion

In this article, a simple, quantitative, liquid phase affinity capture assay is presented. It is a reliable technique based on the interaction between magnetic beads and tagged proteins (e.g. nanobodies) on one hand and the affinity between the tagged protein and a second, labeled protein (e.g. poliovirus) on the other.

Utilizamos cookies para mejorar su experiencia en nuestra página web.

Al continuar usando nuestro sitio web o al hacer clic en 'Continuar', está aceptando nuestras cookies.

Saber más