Expression, Induction, Cell Lysis and DNase I Treatment
5:28
Purification on Amylose Column
7:07
PreScission Protease Cleavage and Cation Exchange Chromatography
8:42
Size Exclusion Chromatography
10:27
Analysis of Purified Mgm101
11:23
Conclusion
The yeast mitochondrial nucleoid protein, Mgm101, is a Rad52-type recombination protein that forms large oligomeric rings. A protocol is described to prepare soluble recombinant Mgm101 using the Maltose Binding Protein (MBP)-tagging strategy coupled with cation exchange and size exclusion chromatography.