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Biology II: Mouse, Zebrafish, and Chick
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The zebrafish (Danio rerio) has become a popular model for studying genetics and developmental biology. The transparency of these animals at early developmental stages permits the direct visualization of tissue morphogenesis at the cellular level. Furthermore, zebrafish are amenable to genetic manipulation, allowing researchers to determine the effect of gene expression on the development of a vertebrate with a high degree of genetic similarity to humans.
This video provides a brief overview of the major phases of zebrafish development, with particular focus on the first 24 hours post fertilization (hpf). The discussion begins with a zygote consisting of a single cell, or blastomere, atop a large ball of yolk. Cleavage of the blastomere is then shown to produce an embryo containing thousands of cells within a matter of hours. Next, the dramatic cellular movements known as epiboly and gastrulation are explained, revealing how they contribute to reshaping a mass of cells into a moving embryo with a beating heart in just 1 day. The presentation follows embryo development through the hatching phase, when they become swimming, feeding larvae. Important considerations for caring for larvae are incorporated, including a brief review of how fish are raised to adulthood in a dedicated facility known as the nursery. Finally, the video concludes with some common techniques utilized for studying embryo development, demonstrating how zebrafish are used to help us better understand human development and disease.
Zebrafish embryos have many characteristics that make them a favorite model among developmental biologists. Their rapid, external development and transparency make them uniquely suited to visualization. Additionally, embryos are amenable to both physical and genetic manipulations, allowing researchers to tease apart the signals controlling developmental processes. This video will go over the zebrafish life cycle, early stages of embryo development, raising fish to adulthood, and highlight some techniques that take advanta
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