Preparation of HeLa-S3 Nuclear Salt-extractable and Chromatin-bound Fractions
7:05
Protein Complexes Purification
11:22
Results: MyoD Interactors Identified by Tandem Affinity Purification Coupled to Mass Spectrometry
13:37
Conclusion
Transcripción
The overall goal of this experiment is to exhaustively characterize MyoD protein partners. This method can answer key questions in the skeletal muscle biology field such as molecular regulation of skeletal muscle tonal deformation by the key muscl
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MyoD is a myogenic transcription factor with a strong capacity to induce myogenic transdifferentiation of many fully differentiated non-muscle cell lines. The epigenetic mechanisms involved in this transdifferentiation are largely unknown. Here we describe a double-affinity purification method followed by mass spectrometry to exhaustively characterize MyoD partners.