Results: 3D Organotypic Model of Human Instestinal Mucosa
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Conclusion
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The overall goal of this procedure is to describe the development of a multi-cellular 3-D organotypic model of the human intestinal mucosa cultured under micro-gravity provided by rotating wall vessel bioreactors. This method has a wide range pote
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Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors.