Preparing and Running the Single-cell Gene-expression Chip
6:52
Data Analysis
8:04
Results: Single-cell Multiplex RT-qPCR Identifies Cell Heterogeneity of the Liver ILC Compartment
9:33
Conclusion
Transcripción
The overall goal of this experiment is to observe multiple gene expression in single cells. This method can help answer key questions in the immunology field, such as heterogenity of molecular signatures within a cell population, or a rare molecul
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This protocol describes how to assess the expression of a large array of genes at the clonal level. Single-cell RT-qPCR produces highly reliable results with a strong sensitivity for hundreds of samples and genes.