Monomeric Avidin Fusion Protein Expression, Purification, and Refolding
3:53
Biotinylation of BCG Cell Surface
5:47
Binding of Monomeric Adivin-fusion Protein to Biotinylated BCG Surface
7:06
Results: Biotinylation with Avidin-OVA
9:13
Conclusion
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The overall goal of this procedure is to express surface proteins on mycobacterium using surface decoration with recombinant proteins as an alternative to traditional, genetic-based approaches. These methods can help answer important research ques
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A novel technique for rapid antigen display on a bacterial surface is presented, which involves surface biotinylation followed by exposure to proteins of interest in fusion with monomeric avidin. Loading BCG with selected antigens successfully improves its immunogenicity, suggesting that surface decoration can replace traditional genetic approaches.