Name: a net mold-based method of scaffold-free three-dimensional cardiac tissue creation
Uploaded: 2018-08-05T13:00:00
Description: Watch this Scientific Journal Video about A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation at JoVE.com

The authors acknowledge the following funding source: the Magic That Matters Fund for Cardiovascular Research.

1. Preparation of Cardiomyocytes
<ol>
	<li>Coat 6-well plates with basement membrane matrix and culture human-induced pluripotent stem cells (hiPSCs) as previously described17.</li>
	<li>Differentiate hiPSCs into hiPSC-CMs using previously described methods18.</li>
	<li>At 16 - 18 d post-differentiation, suspend the cardiomyocytes by rinsing each well with 2 mL of 1x phosphate-buffered saline (PBS) without calcium or magnesium, followed by incubation with 1 mL/well of try...

<ol>
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The significance of this method lies in its reproducibility and the effectiveness of the resultant multilayered cardiac tissue. In the field of cardiac tissue engineering, one of the current goals is to identify a method to construct beating, multilayered, and functional 3-D cardiac patches. We report an efficient and reproducible method of creating multilayered cardiac tissues by direct manual seeding of spheroids composed of cardiomyocytes, endothelial cells, and fibroblasts into a novel net mold. The net mold used in ...

The goal of current cardiac tissue engineering is to develop a therapy to replace or repair the structure and function of injured myocardial tissue1. Methods to create 3-D cardiac tissue models exhibiting the important contractile and electrophysiological properties of native cardiac tissue have been rapidly expanding2,3. A variety of strategies have been explored and used in studies4,5. These methods range from the use of specific synthetic and natural bioactive hydrogels, such as gelatin, collagen, fibrin, and peptides6, to bio-ink deposition technologies2 and bioprinting technologies7. 
It has been shown that scaffold-free methods can produce comparable tissues as biomaterial-based methods, without the drawbacks of incorporating foreign scaffolding material8. Oren Caspi et al. demonstrated that the incorporation of various types of cells enables the generation of highly vascularized human engineered cardiac tissue9. Chin et al. developed a 3-D printing method for cardiac patch creation from spheroids. Resulting patches are composed of cardiomyocytes, fibroblasts, and endothelial cells in a 70:15:15 ratio10. Spheroids have been shown to be effective "building blocks" of scaffold-free cardiac tissue creation, as they are resistant against hypoxia and possess sufficient mechanical integrity for implantation11,12. Previous studies have demonstrated several fabrication methods for spheroid creation, including the use of the hanging drop method, spinner flasks13, microfluidic systems14, and non-adherent culture surfaces uncoated or coated with agarose micro-molds15. In this protocol, we use the hanging drop device, which contains micropores for condensing hundreds of spheroids at one time.
This study presents a novel and efficient scaffold-free method for cardiac tissue creation, which includes manually seeding the spheroids into a square mold cavity and incubating the tissue on a shaker for maturation. Under usual static culture conditions, oxygen diffusion is limited to the outer aspects of the tissue construct, resulting in central necrosis. However, with the net mold, all the spheroids seeded into the mold are immersed in media with a constant fluidic motion, allowing for the increased diffusion of nutrients and oxygen. Additionally, this mold-based method allows for the simultaneous creation of different-sized tissue patches with minimal manual effort and the resultant tissue can be easily removed from the mold. This novel method allows for the efficient and reproducible creation of scaffold-free, multilayered cardiac patches.

<table border="0" cellpadding="0" cellspacing="0" style="width:708px;" width="708">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Human Cardiac fibroblasts (HCF)</td>
			<td style="width:139px;">Sciencell</td>
			<td style="width:119px;">6310</td>
			<td style="width:220px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">FM-2 Consists of Basal Medium</td>
			<td style="width:139px;">Sciencell</td>
			<td style="width:119px;">2331</td>
			<td style="width:220px;">HCF culture medium</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:231px;">Human umbilical vein endothelial cells (HUVEC)</td>
			<td style="width:139px;">Lonza</td>
			<td style="width:119px;">CC-2935</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">EGM+Bullet Kit&#160;</td>
			<td style="width:139px;">Lonza</td>
			<td style="width:119px;">CC5035</td>
			<td>HUVEC culture medium</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">E8 media&#160;</td>
			<td style="width:139px;">Invitrogen</td>
			<td style="width:119px;">A1517001</td>
			<td>HiPSC culture medium</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Geltrex&#160;</td>
			<td style="width:139px;">Invitrogen</td>
			<td style="width:119px;">A1413202</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">TrypLE Express Enzyme (1X)</td>
			<td style="width:139px;">Thermo Fisher</td>
			<td style="width:119px;">12604013</td>
			<td>Trypsin and Cell dissociation reagent</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:231px;">RPMI media</td>
			<td style="width:139px;">Invitrogen</td>
			<td style="width:119px;">11875093</td>
			<td style="width:220px;">RPMI media with B-27 supplement is hiPSC-CM culture medium</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:231px;">B-27 supplement (50x)</td>
			<td style="width:139px;">Thermo Fisher</td>
			<td style="width:119px;">17504044</td>
			<td style="width:220px;">RPMI media with B-27 supplement is hiPSC-CM culture medium</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Trypan Blue Solution, 0.4%</td>
			<td style="width:139px;">Thermo Fisher</td>
			<td style="width:119px;">15250061</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Novel net mold&#160;</td>
			<td style="width:139px;">TissueByNet Co.,Ltd</td>
			<td style="width:119px;">NM25-1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Hanging drop plate</td>
			<td style="width:139px;">Kuraray Co.,Ltd</td>
			<td style="width:119px;">MPc350</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">6 well plates&#160;</td>
			<td style="width:139px;">Sigma-Aldrich</td>
			<td style="width:119px;">CLS-3516</td>
			<td></td>
		</tr>
	</tbody>
</table>

a net mold-based method of scaffold-free three-dimensional cardiac tissue creation

This protocol describes a novel and easy net mold-based method to create three-dimensional (3-D) cardiac tissues without additional scaffold material. Human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), human cardiac fibroblasts (HCFs), and human umbilical vein endothelial cells (HUVECs) are isolated and used to generate a cell suspension with 70% iPSC-CMs, 15% HCFs, and 15% HUVECs. They are co-cultured in an ultra-low attachment "hanging drop" system, which contains micropores for condensing hundreds of spheroids at one time. The cells aggregate and spontaneously form beating spheroids after 3 days of co-culture. The spheroids are harvested, seeded into a novel mold cavity, and cultured on a shaker in the incubator. The spheroids become a mature functional tissue approximately 7 days after seeding. The resultant multilayered tissues consist of fused spheroids with satisfactory structural integrity and synchronous beating behavior. This new method has promising potential as a reproducible and cost-effective method to create engineered tissues for the treatment of heart failure in the future.

In our experiments, we utilized a cell suspension of 70% iPSC-CMs, 15% HCFs, and 15% HUVECs in RPMI/B-27 cell media at a concentration of 2,475,000 cells per mL. After creating the cell suspension, we dispensed 4 mL of the cell suspension to each well of an ultra-low attachment hanging drop system, as described in step 4.3 of the protocol. The use of the hanging drop system resulted in the spontaneous formation of hundreds of beating spheroids after 3 days of culture at 37 &#176;C, 5% CO<...

Watch this Scientific Journal Video about A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation at JoVE.com

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

This protocol describes a net mold-based method to create three-dimensional scaffold-free cardiac tissues with satisfactory structural integrity and synchronous beating behavior.

This protocol describes a novel and easy net mold-based method to create three-dimensional (3-D) cardiac tissues without additional scaffold material. ...

This protocol describes a novel and easy mold based method for three dimensional cardiac tissue creation, which uses spheroids as a tool for scaffold free cardiac tissue creation. In our experiments three types of cells, human induced pluripotent stem cell-derived cardiomyocytes, human cardiac fibroblasts, and human umbilical vein endothelial cells were isolated and used to generate a cell suspension. With 70%cardiomyocytes, 15%fibroblasts, and 15%endothelial cells, in a 50ML conical tube.We then dispense the cell suspension into each well of an ultra low attachment hanging drop system, which will result in the spontaneous formation of hundreds of beating spheroids after three days. The spheroids are then harvested and seated into a novel mold cavity. The tissue is then maintained in a sterile container with medium, on a shaker in the incubator for several days.The mold is subsequently removed, resulting in a scaffold free tissue patch with mechanical integrity. The hanging drop device is a reusable, ultra low attachment well with 850 micro pores, each with a diameter of 350 microns. The hanging drop is compatible with standard six well plates.After making the cell suspension we dispense four milliliters of cell suspension containing 10 million cells, to each well of the hanging drop system. The devices are cultured at 37 degrees celsius, 5%carbon dioxide and 95%humidity. After 72 hours spontaneous formation of beating spheroids, with an average diameter of 350 microns is observed.The spheroids are harvested by placing the hanging drop device into another dish with medium, and swirling gently to release the spheroids and allow them to drift through the pores at the bottom of the hanging drop system. This is a schematic of the novel mold for three dimensional, scaffold free, cardiac tissue creation. It includes the handling mat, filling base and novel mold.The novel mold is comprised of a base, a bottom square plate, a bottom net, 10 layers of side nets made with fine stainless steel prongs. A top net, a top square plate, the holding tubes, and stopper pins. The Novel Mold system can create different sizes of tissues, including two by two millimeters, four by four millimeters, and six by six millimeters.The base bottom square plate and bottom net are stacked and aligned using the corner posts. 10 layers of side nets are then stacked in alternating directions to create a net of fine stainless steel prongs, with the aid of a pair of sterile forceps. The filling base is then flushed with 1X phosphate buffer solution, or PBS, to reduce the surface tension prior to seating the spheroids into the novel mold.After wetting the novel mold cavity in the same methane the spheroids are seated into the presupposed cavity of desired size. Upon seating the excess medium drains through the net mold, allowing the spheroids to fill and assume the shape of the predefined cavity. The top net and top square plate are then assembled onto the corner posts and the stopper pins and the holding tubes are fixed, to prevent washout of the spheroids.The filled novel mold system is transferred to a six well plate, with six millimeters of B-27 supplemented medium, and then maintained in the incubator on a swinging shaker for seven to ten days, at 30 to 40 RPM. The duration of incubation can be altered, depending on the size of the cardiac patch. After incubation the system is taken out and the holding tube, stoppers, the top square plate and the top net are removed.The layered side nets are then carefully slid out one by one, taking care not to disrupt the tissue. After removal of all side nets, an intact cardiac patch is obtained on top of the bottom net. Functional synchronous beating is observed within 24 hours.This novel mold based method is an efficient and easy method of creating three dimensional scaffold free cardiac tissues, with minimal manual effort. The resulting multi-layered tissues consist of fused spheroids with satisfactory structural integrity and synchronous beating behavior. This novel mold based method presents a cost effective and scalable alternative to current bio-fabrication methods of engineered tissues.

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