Results: Fiji-ImageJ Analysis is Accurate and Precise in Determining Metastatic Burden
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Conclusion
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This protocol quantifies lung metastasis, which is an aggressive and common cause of breast cancer related death, with increased precision and efficiency in a preclinical breast cancer model. Compared to the original technique, this protocol gives
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We describe a more consistent and expeditious method to quantify lung metastasis in the 4T1 breast cancer model by using Fiji-ImageJ.