<meta charset="utf8"></head><body>Bioimpresión 3D para la fabricación rápida de redes vasculares mediante recipientes en una placa

<table><tbody><tr><td>10 mL Serological Pipette</td><td>SPL</td><td>SPL 91010</td><td></td></tr><tr><td>10 mL syringe&amp;nbsp;</td><td>Shinchang Medical</td><td></td><td></td></tr><tr><td>15 mL conical tube</td><td>SPL</td><td>50015</td><td></td></tr><tr><td>3D Bioprinter&amp;nbsp;</td><td>T&amp;amp;R Biofab</td><td>3DX-Printer</td><td></td></tr><tr><td>6-well plate&amp;nbsp;</td><td>SPL</td><td>37206</td><td></td></tr><tr><td>Biological Safety Cabinets</td><td>CHC LAB</td><td>PCHC-777A2-04,&amp;nbsp;</td><td></td></tr><tr><td>Brightfield Inverted Microscopes</td><td>Leica</td><td>DMi1</td><td></td></tr><tr><td>Cell Counting Kit (CCK8)</td><td>GlpBio</td><td>GK10001</td><td></td></tr><tr><td>Cell Counting Kit (CCK8)</td><td>GlpBio</td><td>GK10001</td><td></td></tr><tr><td>Cell Culture Flask 75T</td><td>SPL</td><td>70075</td><td></td></tr><tr><td>Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free, 10 mL</td><td>Corning</td><td>354230</td><td></td></tr><tr><td>Distilled water</td><td></td><td></td><td></td></tr><tr><td>DMEM/F12</td><td>Gibco</td><td>11320033</td><td></td></tr><tr><td>DMSO, Cell Culture Grade</td><td>Sigma aldrich</td><td>D2438</td><td></td></tr><tr><td>Dow-Corning, PDMS-Sylgard 184a Kit</td><td>DOW</td><td>DC-184</td><td></td></tr><tr><td>DOWSIL SE 1700 Clear W/C 1.1 KG Kit&amp;nbsp;</td><td>DOW</td><td>2924404</td><td></td></tr><tr><td>D-PBS - 1x</td><td>Welgene</td><td>LB001-01</td><td></td></tr><tr><td>Endothelial Cell Growth Medium MV 2 (Ready to use)</td><td>Promocell</td><td>C-22022</td><td></td></tr><tr><td>Eppendorf Micro pipette(1000,200,100,20,10)</td><td>eppendorf</td><td></td><td></td></tr><tr><td>Ethyl Alcohol 99.9%</td><td>Duksan</td><td>D5</td><td></td></tr><tr><td>Excel</td><td>Microsoft</td><td></td><td></td></tr><tr><td>Fibrinogen from bovine plasma</td><td>Sigma Aldrich</td><td>F8630-1G</td><td></td></tr><tr><td>FITC Dextran 70 kDa</td><td>Sigma Aldrich</td><td>46945-100MG-F</td><td></td></tr><tr><td>Fluorescent beads (1.0 &amp;mu;m, green)</td><td>Sigma Aldrich</td><td>L1030-1ML</td><td></td></tr><tr><td>GelMA-powder (Gelatin methacrylate) 50 g</td><td>3D Materials&amp;nbsp;</td><td>20JT29</td><td></td></tr><tr><td>Gibco, Recovery Cell Culture Freezing Medium, 50 mL</td><td>Gibco</td><td></td><td></td></tr><tr><td>HUVECs (Human Umbillical Vein Endothelial Cells)</td><td>Promocell</td><td></td><td></td></tr><tr><td>ImageJ software</td><td>NIH</td><td></td><td></td></tr><tr><td>Incubator</td><td>Thermo SCIENTIFIC</td><td>Forma STERI-CYCLE i160 CO&lt;sub&gt;2&lt;/sub&gt; Incubator</td><td></td></tr><tr><td>Invitrogen, Live/dead viability/cytotoxicity Kit (for mammalian cells)</td><td>Thermo Fisher</td><td>L3224</td><td></td></tr><tr><td>Lithium Phenyl (2,4,6-trimethylbenzoyl) phosphate powder&amp;nbsp;</td><td>Tokoyo Chemical Industry CO.</td><td>85073-19-4&amp;nbsp;</td><td></td></tr><tr><td>Marienfeld Superior, Counting chamber cover</td><td>Marienfeld Superior</td><td></td><td></td></tr><tr><td>Marienfeld Superior, Hemocytometer, cell counting chamber</td><td>Marienfeld Superior</td><td>HSU-0650030</td><td></td></tr><tr><td>Microcentrifuge</td><td>eppendorf</td><td>Centrifuge 5920 R</td><td></td></tr><tr><td>NCViewer.com</td><td></td><td></td><td></td></tr><tr><td>Nitrogen tank</td><td>WORTHINGTON INDUSTRIES</td><td>LS750</td><td></td></tr><tr><td>Omnicure UV Laser</td><td>EXCELITAS</td><td>SERIES 1500</td><td></td></tr><tr><td>Parafilm M</td><td>amcor</td><td>PM-996</td><td></td></tr><tr><td>Penicillin-Streptomycin Solution (100x)</td><td>GenDEPOT</td><td>CA005-010</td><td></td></tr><tr><td>Planetary Mixer</td><td>THINKY CORPORATION, japan</td><td>ARE-310</td><td></td></tr><tr><td>Plasma treatment machine</td><td>FEMTO SCIENCE</td><td>CUTE-1MPR</td><td></td></tr><tr><td>Pluronic F-127</td><td>Sigma aldrich</td><td>P2443-250G</td><td></td></tr><tr><td>Pre-made buffer, (P2007-1) 10x PBS</td><td>Biosesang</td><td>PR4007-100-00</td><td></td></tr><tr><td>Reagent storage cabinet</td><td>ZIO FILTER TECH</td><td>SC2-30F-1306D1-BC</td><td></td></tr><tr><td>Real time Live cell Imaging Microscope</td><td>Carl ZEISS</td><td></td><td></td></tr><tr><td>Refrigerator</td><td>SAMSUNG</td><td>RT50K6035SL</td><td></td></tr><tr><td>ROCKER 2D digital</td><td>IKA</td><td>4003000</td><td></td></tr><tr><td>Scoop-Spatula</td><td>CacheBy</td><td>SL-SCO7001-EA</td><td></td></tr><tr><td>sigma,Trypsin-EDTA solition, 0.25%</td><td>Sigma aldrich</td><td>T4049-100ML</td><td></td></tr><tr><td>Sodium Dodecyl Sulfate (SDS)</td><td>Thermo Fisher scientific</td><td>151-21-3</td><td></td></tr><tr><td>Syringe Barrel Tip Cap</td><td>FISNAR</td><td>3051806</td><td></td></tr><tr><td>Tally counter</td><td>Control Company</td><td>&amp;nbsp;C23-147-050</td><td></td></tr><tr><td>Tapered Nozzle (18 G)</td><td>Mushashi</td><td>TPND-18G-U</td><td></td></tr><tr><td>Tapered Nozzle (22 G)</td><td>Mushashi</td><td>TPND-22G-U</td><td></td></tr><tr><td>Tapered nozzle 20 G</td><td>Musashi</td><td>TPND-20G-U</td><td></td></tr><tr><td>Thrombin from bovine plasma</td><td>Sigma Aldrich</td><td>T7326-1KU</td><td></td></tr><tr><td>Timer, 4-channel</td><td>ETL</td><td>SL.Tim3005</td><td></td></tr><tr><td>Trypan Blue Solution 0.4%</td><td>Gibco</td><td>15250061</td><td></td></tr><tr><td>Trypsin Neutralizing Solution</td><td>Promocell</td><td>C-41120</td><td></td></tr><tr><td>UG 24 mL UG ointment jar</td><td>Yamayu</td><td>No. 3-53</td><td></td></tr><tr><td>UG 58 mL UG ointment jar</td><td>Yamayu</td><td>No. 3-55</td><td></td></tr><tr><td>Water Bath</td><td>DAIHAN Scientific</td><td>WB-11</td><td></td></tr><tr><td>Weight machine</td><td>Sartorius</td><td>bce2241-1skr</td><td></td></tr></tbody></table>

high-throughput bioprinting method for modeling vascular permeability in standard six-well plates with size and pattern flexibility

<meta charset="utf8"></head><body>Autor destacado: Bioimpresión automatizada para la fabricación de modelos vasculares de alto rendimiento

Método de bioimpresión de alto rendimiento para modelar la permeabilidad vascular en placas estándar de seis pocillos con flexibilidad de tamaño y patrón

Vascular permeability is a key factor in developing therapies for disorders associated with compromised endothelium, such as endothelial dysfunction in ...

high-throughput-bioprinting-method-for-modeling-vascular-permeability

Usage Statistics

Top Institutions