S'identifier

The Johns Hopkins University

7 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Preparation of Mouse Pituitary Immunogen for the Induction of Experimental Autoimmune Hypophysitis
Shey-Cherng Tzou 1, Melissa A. Landek-Salgado 1, Hiroaki Kimura 1, Patrizio Caturegli 1
1Department of Pathology, The Johns Hopkins University

Autoimmune hypophysitis can be reproduced in mice by injecting an extract of mouse pituitary proteins.

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Immunology and Infection

Induction of Experimental Autoimmune Hypophysitis in SJL Mice
Melissa A. Landek-Salgado 1, Shey-Cherng Tzou 1, Hiroaki Kimura 1, Patrizio Caturegli 1
1Department of Pathology, The Johns Hopkins University

This video shows how to induce autoimmune hypophysitis in SJL mice and how to assess its severity by histopathology.

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Medicine

Quantitative Visualization and Detection of Skin Cancer Using Dynamic Thermal Imaging
Cila Herman 1, Muge Pirtini Cetingul 1
1Department of Mechanical Engineering, The Johns Hopkins University

We demonstrated that malignant pigmented lesions with increased metabolic activity generate quantifiable amounts of heat and the measurement of the transient thermal response of the skin to a cooling excitation allows quantitative identification of melanoma and other skin cancers (vs. non-proliferative nevi) at an early stage of the disease.

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Biology

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue
Vuong Tran 1, Qiang Gan 1, Xin Chen 1
1Department of Biology, Johns Hopkins University

Recently high-throughput sequencing technology has greatly increased sensitivity of Chromatin Immunoprecipitation (ChIP) experiment and prompted its application using purified cells or dissected tissue. Here we delineate a method to use ChIP technique with Drosophila tissue, which can address the endogenous chromatin state in a well-characterized biological system.

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Biology

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Derek C. Prosser 1, Kristie Wrasman 1, Thaddeus K. Woodard 1, Allyson F. O’Donnell 2, Beverly Wendland 1
1Department of Biology, The Johns Hopkins University, 2Department of Biological Sciences, Duquesne University

Accurate quantification of vesicular trafficking events often provides key insights into roles for specific proteins and the effects of mutations. This paper presents methods for using superecliptic pHluorin, a pH-sensitive GFP variant, as a tool for quantification of endocytic events in living cells using quantitative fluorescence microscopy and flow cytometry.

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Biology

Super-Resolution Live Cell Imaging of Subcellular Structures
Rajesh Ranjan 1, Xin Chen 1
1Department of Biology, The Johns Hopkins University

Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live tissue.

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Neuroscience

A Standardized Pipeline for Examining Human Cerebellar Grey Matter Morphometry using Structural Magnetic Resonance Imaging
Rebecca Kerestes 1, Shuo Han 2, Srinivas Balachander 3, Carlos Hernandez-Castillo 4, Jerry L. Prince 5,6, Jörn Diedrichsen 7, Ian H. Harding 1,8
1Department of Neuroscience, Central Clinical School, Monash University, 2Department of Biomedical Engineering, The Johns Hopkins University, 3Department of Psychiatry, National Institute of Mental Health & Neuro Sciences (NIMHANS), 4Faculty of Computer Science, Dalhousie University, 5Department of Electrical and Computer Engineering, The Johns Hopkins University, 6Department of Computer Science, The Johns Hopkins University, 7Brain and Mind Institute, Department for Statistical and Actuarial Sciences, Department for Computer Science, Western University, 8Monash Biomedical Imaging, Monash University

A standardized pipeline is presented for examining cerebellum grey matter morphometry. The pipeline combines high-resolution, state-of-the-art approaches for optimized and automated cerebellum parcellation and voxel-based registration of the cerebellum for volumetric quantification.

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