S'identifier

Germany & Joint Heidelberg-IDC Translational Diabetes Program

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

Asa S. Hidmark¹, Peter P. Nawroth^1,2,3, Thomas Fleming^1,3

¹Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, ²Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, ³German Center for Diabetes Research (DZD)

Quantitative analysis of cell content within the murine sciatic nerve is difficult due to the scarcity of the tissue. This protocol describes a method for tissue digestion and preparation that provides sufficient cells for flow cytometry analysis of immune cell populations from nerves of individual mice.