JOVE JOURNAL

Immunologie et infection

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The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood

Journal (Immunology and Infection) | Methods Collections

The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood

Anjali S. Yennemadi¹, Joseph Keane¹, Gina Leisching¹

¹TB Immunology Group, Department of Clinical Medicine, Trinity Translational Medicine Institute, St James's Hospital, Trinity College Dublin, The University of Dublin

Prion Safety Laboratory Swipe Test

Journal (Immunology and Infection)

Prion Safety Laboratory Swipe Test

Sara M. Simmons¹, Qi Yuan¹, Jason C. Bartz¹

¹Department of Medical Microbiology and Immunology, School of Medicine, Creighton University

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Journal (Immunology and Infection) | Methods Collections

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Katherine Do¹, Subina Mehta¹, Reid Wagner², Timothy J. Griffin¹, Pratik D. Jagtap¹

¹Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, ²Minnesota Supercomputing Institute, University of Minnesota

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Journal (Immunology and Infection) | Methods Collections

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Kathryn M. Crotty¹^,², Samantha M. Yeligar¹^,²

¹Department of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Emory University, ²Atlanta Veterans Affairs Health Care System

Antimicrobial Activity and Toxicity Assessment of Compounds Targeting <i>Mycobacterium abscessus</i> in Macrophage Infection Assays

Journal (Immunology and Infection)

Antimicrobial Activity and Toxicity Assessment of Compounds Targeting Mycobacterium abscessus in Macrophage Infection Assays

Gabriel S. Oliveira¹^,², Clara M. Bento¹^,³^,⁴, António Pombinho¹^,³, Rita Reis¹^,³, Kevin Van Calster⁵^,⁶, Linda De Vooght⁵, André F. Maia¹^,³, Paul Cos⁵, Maria Salomé Gomes¹^,², Tânia Silva¹^,²

¹i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, ²ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ³IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, ⁴Programa Doutoral em Biologia Molecular e Celular (MCBiology), Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, ⁵Laboratory for Microbiology, Parasitology and Hygiene (LMPH), ⁶Department of Infectious Diseases in Humans, Immune Response, Sciensano

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Journal (Immunology and Infection) | Methods Collections

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Zejin Cui*¹, Pengkun Yuan*², Zhishan Zhao*¹, Fan Zhao³, Linrong Lu¹^,³

¹Shanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ²Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, ³Institute of Immunology and Department of Rheumatology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Journal (Immunology and Infection) | Methods Collections

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Jaya Lakshmi Thangaraj¹, Dan S. Kaufman¹^,²

¹Department of Medicine, Division of Regenerative Medicine, University of California, San Diego, ²Sanford Stem Cell Institute, University of California, San Diego

Generation of Human Chimeric Antigen Receptor Regulatory T Cells

Journal (Immunology and Infection)

Generation of Human Chimeric Antigen Receptor Regulatory T Cells

Russell W. Cochrane¹^,²^,³, Rob A. Robino¹^,²^,³, Leonardo M. R. Ferreira¹^,²^,³

¹Department of Microbiology and Immunology, Medical University of South Carolina, ²Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, ³Hollings Cancer Center, Medical University of South Carolina

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Journal (Immunology and Infection) | Methods Collections

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Gabriel S. Oliveira¹^,², Clara M. Bento¹^,³^,⁴, António Pombinho¹^,³, Rita Reis¹^,³, Kevin Van Calster⁵^,⁶, Linda De Vooght⁵, André F. Maia¹^,³, Paul Cos⁵, Maria Salomé Gomes¹^,², Tânia Silva¹^,²

¹i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, ²ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ³IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, ⁴Programa Doutoral em Biologia Molecular e Celular (MCBiology), Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, ⁵Laboratory for Microbiology, Parasitology and Hygiene (LMPH), ⁶Department of Infectious Diseases in Humans, Immune Response, Sciensano

Efficient Method for Imaging Murine Lungs that Preserves Spatial Dynamics of Fungal Spores in the Airways

Journal (Immunology and Infection) | Methods Collections

Efficient Method for Imaging Murine Lungs that Preserves Spatial Dynamics of Fungal Spores in the Airways

Paul J. Brennan¹, Nicolas W. S. Caballes¹, Anjanique Mercado-Feliciano¹, Yen-Fei Wu¹, Darin L. Wiesner¹

¹Department of Medicine, New Jersey Medical School, Rutgers - The State University of New Jersey

Preparation of Whole Blood Samples for Flow Cytometry and Cytokine Analysis to Determine Antigen-Specific Host Immunity to Fungal and Viral Pathogens

Journal (Immunology and Infection)

Preparation of Whole Blood Samples for Flow Cytometry and Cytokine Analysis to Determine Antigen-Specific Host Immunity to Fungal and Viral Pathogens

Lukas Page¹, Chris D. Lauruschkat², Kevin Dennehy¹, Eva Loell¹, Beeke Tappe², Andre Fuchs³, Sebastian Wurster*⁴, Reinhard Hoffmann*¹

¹Institute for Laboratory Medicine and Microbiology, University Hospital Augsburg, ²Department of Internal Medicine II, University Hospital of Wuerzburg, ³Internal Medicine III - Gastroenterology and Infectious Diseases, University Hospital Augsburg, ⁴Department of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center, University of Texas Houston

Stimulation and Incubation of Whole Blood Samples for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens

Journal (Immunology and Infection)

Stimulation and Incubation of Whole Blood Samples for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens

Lukas Page¹, Chris D. Lauruschkat², Kevin Dennehy¹, Eva Loell¹, Beeke Tappe², Andre Fuchs³, Sebastian Wurster*⁴, Reinhard Hoffmann*¹

¹Institute for Laboratory Medicine and Microbiology, University Hospital Augsburg, ²Department of Internal Medicine II, University Hospital of Wuerzburg, ³Internal Medicine III - Gastroenterology and Infectious Diseases, University Hospital Augsburg, ⁴Department of Infectious Diseases, Infection Control and Employee Health, MD Anderson Cancer Center, University of Texas Houston

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