Gyeongsang National University

4 ARTICLES PUBLISHED IN JoVE

Biology

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Ritesh Kumar^*1, Shu Wei Wu^*1, Arya Bagus Boedi Iswanto¹, Dhinesh Kumar¹, Xiao Han², Jae-Yean Kim¹

¹Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, ²Biotechnology Research Institute, Chinese Academy of Agricultural Sciences

This article describes the methods for screening the genes controlling plasmodesmal permeability and hence auxin gradient during tropic response. This includes the measurement of the degree of tropic response in hypocotyl of Arabidopsis thaliana and checking plasmodesmal permeability by 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) loading and finally callose level assessment.

Medicine

Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs

Won-Jae Lee^1,2, Ji-Sung Park¹, Si-Jung Jang¹, Seung-Chan Lee¹, HyeonJeong Lee¹, Jae-Hoon Lee¹, Gyu-Jin Rho¹, Sung-Lim Lee¹

¹College of Veterinary Medicine, Gyeongsang National University, ²PWG Genetics Pte Ltd.

A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.

Chemistry

Synthesis of Plant Phenol-derived Polymeric Dyes for Direct or Mordant-based Hair Dyeing

Kyung Min Im¹, Jong-Rok Jeon¹

¹Department of Agricultural Chemistry and Food Science & Technology, Institute of Agriculture & Life Science, Gyeongsang National University

Here, we present a protocol to use pre-synthesized polymeric products derived from fungal laccase-catalyzed polymerization of plant phenols, either with or without mordant agents (e.g., FeSO₄), to induce detergent-resistant keratin hair dyeing within 2.5 hours.

Bioengineering

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

Rong Li^*1, Jinxin Miao^*1,2, Zhiqiang Fan¹, SeokHwan Song³, Il-keun Kong^3,4, Yaohe Wang^2,5, Zhongde Wang¹

¹Department of Animal, Dairy, and Veterinary Sciences, Utah State University, ²National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, ³Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, ⁴Institute of Agriculture and Life Science, Gyeongsang National University, ⁵Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London

Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system.