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Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs

DOI :

10.3791/54077-v

July 2nd, 2016

July 2nd, 2016

10,113 Views

1College of Veterinary Medicine, Gyeongsang National University, 2PWG Genetics Pte Ltd.

A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs.

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