Nanjing Tech University

8 ARTICLES PUBLISHED IN JoVE

Chemistry

Using Polystyrene-block-poly(acrylic acid)-coated Metal Nanoparticles as Monomers for Their Homo- and Co-polymerization

Yawen Wang¹, Xiohui Song¹, Hong Wang¹, Hongyu Chen¹

¹Division of Chemistry and Biological Chemistry, Nanyang Technological University

We report protocols for “polymerizing” various types of polymer-encapsulated metal nanoparticles into long chains of “homo-“ and “co-polymers”.

Biochemistry

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Zhihui Xu^*1, Yiyan Song^*1, Huijun Jiang², Yan Kong³, Xiaoming Li³, Jin Chen¹, Yuan Wu⁴

¹School of Public Health, Nanjing Medical University, ²School of Pharmacy, Nanjing Medical University, ³State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, ⁴Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University

This protocol describes a general strategy to regenerate commercial arrayed gold microelectrodes equipped for a label-free cell analyzer aimed at saving on the high running costs ofmicrochip-based assays. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step, which enables repeated usage of microchips.

Biology

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Feng Pan^*2, Jie Zhang^*3, Yuyan Liu¹, Liangsheng Lu¹, Xuefeng Qiu⁴, Kangtai Lv⁵, Qipeng Zhang¹

¹State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, Nanjing University, ²Department of Andrology, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, ³School of Pharmaceutical Sciences, Nanjing Tech University, ⁴Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, ⁵Department of Ultrasound, Nanjing Qixia District Maternity and Child Health Care Hospital

Intracavernosal pressure recording (ICP) is an important method to evaluate the erectile function of experimental animals. Here, a detailed protocol is demonstrated for the recording procedure of ICP by catheterizing the crura penis and then electrically stimulating the cavernous nerves in rats.

Chemistry

Synthesis of Substrate-Bound Au Nanowires Via an Active Surface Growth Mechanism

Xinglong Wang^*1, Xuesong Wu^*1, Jiating He², Xiaolin Tao¹, Hongyan Li¹, Gui Zhao¹, Yawen Wang¹, Hongyu Chen^1,2

¹Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, ²Chemistry and Biological Chemistry, Nanyang Technological University

We report a solution-based method to synthesize substrate-bound Au nanowires. By tuning the molecular ligands used during the synthesis, the Au nanowires can be grown from various substrates with different surface properties. Au nanowire-based nanostructures can also be synthesized by adjusting the reaction parameters.

Medicine

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Di Liu^*1, Xizhu Xu^*2, Yuejin Li², Jie Zhang¹, Xiaoyu Zhang¹, Qihuan Li¹, Haifeng Hou², Dong Li², Wei Wang^1,2,3, Youxin Wang¹

¹Beijing Key Laboratory of Clinical Epidemiology, School of Public Health, Capital Medical University, ²School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, ³School of Medical and Health Sciences, Edith Cowan University

Immunoglobulin G (IgG) N-glycan is characterized using hydrophilic interaction chromatography UPLC. In addition, the structure of IgG N-glycan is clearly separated. Presented here is an introduction to this experimental method so that it can be widely used in research settings.

JoVE Core

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

Jie Zhang^1,2, Fang Fang^1,3, Liang Li¹, Haoliang Huang¹, Hannah C. Webber¹, Yang Sun^1,4, Vinit B. Mahajan^1,4, Yang Hu¹

¹Department of Ophthalmology, Stanford University School of Medicine, ²Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ³Department of Ophthalmology, Second Xiangya Hospital of Central South University, ⁴Department of Ophthalmology, Veterans Affairs Palo Alto Health Care

Here, we present a protocol to induce ocular hypertension and glaucomatous neurodegeneration in mouse eyes by intracameral injection of silicone oil and the procedure for silicone oil removal from the anterior chamber to return elevated intraocular pressure to normal.

Biology

Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Jinxi Yuan¹, Jie Zhang¹, Yan Zhang¹, WuYun QiQiGe¹, Wei Liu², Shanchun Yan¹, Guirong Wang²

¹Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, ²Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

Bioengineering

Isolation, Purification, and Identification of Bacitracin-producing Bacillus licheniformis from Fresh Feces of Healthy Pigs

Yushu Tang¹, Ning Hao¹

¹College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University

This protocol provides a complete and comprehensive description of the detailed process for the isolation, purification, and identification of bacitracin-producing Bacillus licheniformis from healthy pig feces.