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Otto von Guericke University Magdeburg

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
Martin Wagner 1, Helen Koester 1, Christian Deffge 1, Soenke Weinert 1, Johannes Lauf 1, Alexander Francke 2, Jerry Lee 3, R. C. Braun- Dullaeus 1, Joerg Herold 1
1Department for Cardiology, Angiology and Pneumology, Otto von Guericke University Magdeburg, 2Herzzentrum Dresden, Universitätsklinikum an der Technischen Universität Dresden, Technische Universität Dresden, 3Department of Public Health and Primary Care, University of Cambridge

Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).

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Medicine

Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease
Martin Wagner 1, Claudia Baer 1, Werner Zuschratter 2, Monika Riek-Burchardt 3, Christian Deffge 1, Soenke Weinert 1, Jerry C Lee 4, Ruediger C Braun-Dullaeus 1, Joerg Herold 1
1Department of Cardiology and Angiology, University of Magdeburg, 2Leibniz Institute for Neurobiology, 3Institute of Molecular and Clinical Immunology, University of Magdeburg, 4Division of Hematology/Oncology, Boston Children's Hospital, Harvard Medical School

Monocytes are important mediators of arteriogenesis in the context of peripheral arterial disease. Using a basement membrane-like matrix and intravital microscopy, this protocol investigates monocyte homing and tumor-related angiogenesis after monocyte injection in the femoral artery ligation murine model.

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Neuroscience

An Unbiased Approach of Sampling TEM Sections in Neuroscience
Stefan Wernitznig 1, Florian Reichmann 2, Mariella Sele 1, Christoph Birkl 3, Johannes Haybäck 4,5, Florian Kleinegger 4, Anna Birkl-Töglhofer 4, Stefanie Krassnig 4, Christina Wodlej 4, Peter Holzer 2, Daniel Kummer 1, Elisabeth Bock 1, Gerd Leitinger 1
1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center, Medical University of Graz, 3Division of General Neurology, Department of Neurology, Medical University of Graz, 4Institute of Pathology, Medical University of Graz, 5Department of Pathology, Medical Faculty, Otto von Guericke University Magdeburg

We introduce a novel workflow for electron microscopy investigations of brain tissue. The method allows the user to examine neuronal features in an unbiased fashion. For elemental analysis, we also present a script that automatizes most of the workflow for randomized sampling.

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Engineering

Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios
Burkhard Hoppenstedt 1, Thomas Probst 2, Manfred Reichert 1, Winfried Schlee 3, Klaus Kammerer 1, Myra Spiliopoulou 4, Johannes Schobel 1, Michael Winter 1, Anna Felnhofer 5, Oswald D. Kothgassner 6, Rüdiger Pryss 7
1Institute of Databases and Information Systems, Ulm University, 2Department for Psychotherapy and Biopsychosocial Health, Danube University Krems, 3Department of Psychiatry and Psychotherapy, University of Regensburg, 4Faculty of Computer Science, Otto von Guericke University Magdeburg, 5Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 6Department of Child and Adolescent Psychiatry, Medical University of Vienna, 7Institute of Clinical Epidemiology and Biometry, University of Würzburg

This protocol delineates the technical setting of a developed mixed reality application that is used for immersive analytics. Based on this, measures are presented, which were used in a study to gain insights into usability aspects of the developed technical solution.

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Biochemistry

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
Laura K. Hillert-Richter 1, Inna N. Lavrik 1
1Translational Inflammation Research, Center of Dynamic Systems, Otto von Guericke University Magdeburg

Here, an experimental workflow is presented that enables the detection of caspase-8 processing directly at the death-inducing signaling complex (DISC) and determines the composition of this complex. This methodology has broad applications, from unraveling the molecular mechanisms of cell death pathways to the dynamic modeling of apoptosis networks.

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