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An Unbiased Approach of Sampling TEM Sections in Neuroscience

DOI :

10.3791/58745-v

10:56 min

April 13th, 2019

April 13th, 2019

6,716 Views

1Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 2Department of Pharmacology, Otto Loewi Research Center, Medical University of Graz, 3Division of General Neurology, Department of Neurology, Medical University of Graz, 4Institute of Pathology, Medical University of Graz, 5Department of Pathology, Medical Faculty, Otto von Guericke University Magdeburg

We introduce a novel workflow for electron microscopy investigations of brain tissue. The method allows the user to examine neuronal features in an unbiased fashion. For elemental analysis, we also present a script that automatizes most of the workflow for randomized sampling.

Tags

Keywords Unbiased Sampling Electron Microscopy Neuroscience Quantification Numerical Density Synapses Neural Network Sample Preparation Ultramicrotome Serial Sections Slot Grid Counterstaining Transmission Electron Microscope TEM TEM Serial Section Software Region Of Interest ROI Random Point Sampling

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