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University of Fribourg, Fribourg, Switzerland

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Induction of Myocardial Infarction in Adult Zebrafish Using Cryoinjury
Fabian Chablais 1, Anna Jaźwińska 1
1Department of Biology, Unit of Zoology, University of Fribourg, Fribourg, Switzerland

Zebrafish represents a valuable model to study the mechanisms of heart regeneration in vertebrates. Here, we present a protocol for induction of a heart infarct in adult zebrafish using cryoinjury. This method results in massive cell death within 20% of the ventricular wall, similar to that observed in mammalian infarcts.

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Biology

Intrathoracic Injection for the Study of Adult Zebrafish Heart
Thomas Bise 1, Anna Jaźwińska 1
1Department of Biology, University of Fribourg

This method relies on the injection of 0.5−3 μL of solution into the thorax of adult zebrafish. The procedure efficiently delivers proteins and chemical compounds into the proximity of the zebrafish heart without damaging the organ. The approach is suitable for testing effects of exogenous factors on various tissues of the heart.

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