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University of Naples Federico II

9 ARTICLES PUBLISHED IN JoVE

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Behavior

Assessing the Influence of Personality on Sensitivity to Magnetic Fields in Zebrafish
Alessandro Cresci 1, Rosario De Rosa 2,3, Claudio Agnisola 4
1Rosenstiel School of Marine and Atmospheric Science, Department of Ocean Sciences, University of Miami, 2Department of Physics, Monte S. Angelo (MSA) Campus, University of Naples Federico II, 3Istituto Nazionale di Fisica Nucleare (INFN), 4Department of Biology, MSA Campus, University of Naples Federico II

We describe a behavioral protocol designed to assess how zebrafish’s personalities influence their response to water currents and weak magnetic fields. Fishes with the same personalities are separated based on their explorative behavior. Then, their rheotactic orientation behavior in a swimming tunnel with a low flow rate and under different magnetic conditions is observed.

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Biochemistry

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
Roberta Imperatore 1,2, Livia D'Angelo 3, Paolo De Girolamo 3, Luigia Cristino 2, Marina Paolucci 1
1Department of Sciences and Technologies (DST), University of Sannio, 2Endocannabinoid Research Group, Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions, University of Naples Federico II

Presented here are protocols for immunohistochemical characterization and localization of orexin peptide, orexin receptors, and endocannabinoid receptors in the gut and brains of normal and diet-induced obesity (DIO) adult zebrafish models using immunoperixidase and double immunofluorescence methods.

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Immunology and Infection

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
Chiara Pagliuca 1, Elena Scaglione 1, Francesca Carraturo 1, Giuseppe Mantova 1, Maria Michela Marino 1, Maria Virginia Pishbin 1, Caterina Pagliarulo 2, Roberta Colicchio 1, Paola Salvatore 1,3
1Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 2Department of Science and Technology, Sannio University, 3CEINGE – Advanced Biotechnology

Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.

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Bioengineering

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
Immacolata Belviso *1, Anna Maria Sacco *1, Veronica Romano 1, Fabrizio Schonauer 1, Daria Nurzynska 1, Stefania Montagnani 1, Franca Di Meglio 1, Clotilde Castaldo 1
1Department of Public Health, University of Naples Federico II

Cell reprogramming requires the introduction of key genes, which regulate and maintain the pluripotent cell state. The protocol described enables the formation of induced pluripotent stem cells (iPSCs) colonies from human dermal fibroblasts without viral/integrating methods but using non-modified RNAs (NM-RNAs) combined with immune evasion factors reducing cellular defense mechanisms.

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Immunology and Infection

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
Sonia Simonetti *1,2, Ambra Natalini *1,2, Giovanna Peruzzi 3, Alfredo Nicosia 4, Antonella Folgori 5, Stefania Capone 5, Angela Santoni 2,6, Francesca Di Rosa 1
1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed

Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated mice.

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Chemistry

Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
Roberta Teta 1, Germana Esposito 1, Carlo De Sterlich 2, Massimiliano Lega *3, Valeria Costantino *1
1TheBlueChemistryLab, Department of Pharmacy, University of Naples Federico II, 2Department of Public Health, University of Naples Federico II, 3Department of Engineering, University Parthenope

A fast-multidisciplinarystrategy for early detection of cyanobacterial blooms and associated cyanotoxins is described here. It allows for the detection of cyanobacteria and related cyanotoxins in water samples and in organic matrices, such as bivalve samples, in 24 h.

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Environment

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Giovanni Gallo 1,2, Martina Aulitto 1,3, Patrizia Contursi 1,4, Danila Limauro 1,4, Simonetta Bartolucci 1, Gabriella Fiorentino 1,4
1Department of Biology, University of Naples Federico II, 2Institute of Polymers, Composites and Biomaterials (IPCB), Consiglio Nazionale delle Ricerche CNR, 3Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, 4BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli Federico II

The isolation of heavy metal-resistant microbes from geothermal springs is a hot topic for the development of bioremediation and environmental monitoring biosystems. This study provides a methodological approach for isolating and identifying heavy metal tolerant bacteria from hot springs.

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Engineering

A Single-Channel and Non-Invasive Wearable Brain-Computer Interface for Industry and Healthcare
Pasquale Arpaia 1,2, Antonio Esposito 1,3, Nicola Moccaldi 1,4, Marco Parvis 5
1Department of Electrical Engineering and Information Technology (DIETI), University of Naples Federico II, 2Interdepartmental Center for Research and Innovation in Management and Healthcare (CIRMIS), University of Naples Federico II, 3Advanced Metrological Services Center (CeSMA), University of Naples Federico II, 4Department of Innovation Engineering, University of Salento, 5Department of Electronics and Telecommunication (DET), Polytechnic of Turin

This paper discusses how to build a brain-computer interface by relying on consumer-grade equipment and steady-state visually evoked potentials. For this, a single-channel electroencephalograph exploiting dry electrodes was integrated with augmented reality glasses for stimuli presentation and output data visualization. The final system was non-invasive, wearable, and portable.

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Biology

Production of Cardiac Extracellular Matrix from Adult Human Fibroblasts for Culture Dish Coating
Daria Nurzynska 1, Anna Maria Sacco 2, Valentino Servodio 2, Veronica Romano 2, Immacolata Belviso 2, Clotilde Castaldo 2, Franca Di Meglio 2
1Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 2Department of Public Health, University of Naples Federico II

Fibroblasts isolated from the adult human heart were cultured to confluence on gelatin-coated dishes to produce the myocardium-specific extracellular matrix. After decellularization, this substrate can be used for the culture and study of other cardiac cells and cell-matrix interactions.

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