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Intestinal Epithelial Monolayers Derived from Established Murine Colonoids (Video) | JoVE

DOI :

10.3791/200278-v

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June 2nd, 2023

June 2nd, 2023

•

106 Views

Erin Crawford^1*, Heather L. Mentrup^2,3*, Elizabeth A. Novak^2,3, Kevin P. Mollen^2,3

¹Division of Pediatric Gastroenterology, Hepatology and Nutrition, UPMC Children’s Hospital of Pittsburgh, ²Division of Pediatric Surgery, UPMC Children’s Hospital of Pittsburgh, ³Department of Surgery, University of Pittsburgh School of Medicine

* These authors contributed equally

Tags

Keywords Intestinal Epithelial Monolayers

Murine Colonoids

Basement Membrane Matrix

Murine Monolayer Medium

Cell Culture Insert

Enzymatic Dissociation Reagent

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