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Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

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02:58 min

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January 5th, 2024

DOI :

10.3791/201505-v

January 5th, 2024

•
47 Views
1Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences, Hiroshima University

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Epitope Tag
Protein Purification
Immunoblotting
Protein protein Interaction
Centrifugation
Lysis Buffer
Sample Buffer
SDS PAGE
PVDF Membrane
Primary Antibody
DYKDDDDK PRDM16
HA EHMT1
HEK 293 Cells

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Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation
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