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Analyzing Mitochondrial Supercomplexes in Mammalian Tissue Fractions via In-Gel Activity Assays

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02:17 min

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May 3rd, 2024

DOI :

10.3791/201794-v

May 3rd, 2024

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1Department of Biochemistry, University of Zaragoza, 2Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza

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Mitochondrial Supercomplexes
In gel Activity Assays
Mammalian Tissue Fractions
Blue Native Sample Buffer
Digitonin
Coomassie Blue G 250
Polyacrylamide Gel Electrophoresis
In gel Activity Assay
Complex I
Complex IV
SCAF1
Mouse Cells
Human Cells

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Mitochondrial Fractionation in Small Tissue Samples for Supercomplex Analysis
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