Aniline Blue Staining to Visualize Plasmodesmata in Agroinfiltrated Plant Leaves Using Confocal Microscopy

To begin, obtain Nicotiana benthamiana leaves infiltrated with the agrobacterium tumefacien cells harboring desired vectors. For visualization of plasmodesmata, add an aliquot of 200 microliters of 1%aniline blue onto a microscope slide. Then, usi