Human ES Cell Differentiation toward the Definitive Endoderm
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CXCR4+ Definitive Endoderm (DE) Cell Immunostaining for Flow Cytometric Analysis
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CXCR4+ Cell Magnetic Bead Isolation
6:34
Results: Representative Changes in Gene/Protein Expression after Human ESC into DE Differentiation
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Conclusion
Transcription
The overall goal of this method is to purify endodermal cells generated from human embryonic stem or ES cells. This method can help answer key questions in the stem cell field about endodermal differentiation. The main advantage of this technique
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Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations.