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Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

9.7K Views

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09:21 min

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January 4th, 2016

DOI :

10.3791/53659-v

January 4th, 2016

•
9,748 Views
1Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, 2Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), 3Department of Biomedical Engineering, Eindhoven University of Technology

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Keywords Genetically Encoded FRET based Bile Acid Sensor
Bile Acid Transport
Bile Acid Dynamics
Glucose Metabolism
Lipid Metabolism
Energy Metabolism
FXR Nuclear Bile Acid Receptor
Cerulean Fluorophore
Citrine Fluorophore
Nuclear Localization Signal
Cytoplasmic Localization
Live Cell Imaging
Leibovitz L 15 Culture Medium
DMEM With Phenol Red
PH Sensitivity
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