Quantitative Tissue and Standard Histology Preparation
4:22
Tissue and Standard Sectioning
5:47
Results: Representative Ex Vivo Whole Organ Fluorescence and Quantitative Tissue Histology Analyses
7:15
Conclusion
Transcription
The overall goal of this procedure is to rapidly characterize the bio-distribution of fluorescently labeled compounds in whole animals. This method can be used to define the bio-distribution of protein based therapeutics and to determine the effec
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Ex vivo whole-organ imaging is a rapid method for determining the relative concentrations of fluorescently labeled compounds within and between tissues or treatment groups. Conversely, quantitative fluorescence histology, while more labor intensive, allows for the quantification of the absolute tissue levels of labeled molecules.