The University of Mississippi Medical Center
2 ARTICLES PUBLISHED IN JoVE
Biology
The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules
Jeremy W. D. McGowan 1, Gene L. Bidwell, III 1
1Department of Neurology, The University of Mississippi Medical Center
Ex vivo whole-organ imaging is a rapid method for determining the relative concentrations of fluorescently labeled compounds within and between tissues or treatment groups. Conversely, quantitative fluorescence histology, while more labor intensive, allows for the quantification of the absolute tissue levels of labeled molecules.
Medicine
Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method
Sarah M. Peterson 1, Xuexiang Wang 2, Ashley C. Johnson 1, Ian D. Coate 1, Michael R. Garrett 1,3, Sean P. Didion 1,4
1Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, 2Department of Medicine, Division of Nephrology, Rush University Medical Center, 3Department of Medicine, Division of Nephrology, The University of Mississippi Medical Center, 4Department of Neurology, The University of Mississippi Medical Center
Estimates of whole kidney nephron number are important clinically and experimentally, as there is an inverse association between nephron number and an enhanced risk of renal and cardiovascular disease.Herein, the use of the acid maceration method, which provides fast and reliable estimates of whole kidney nephron number, is demonstrated.
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