Lentivirus Particle Release and Transduction Capacity
3:51
Crtitical Bone Defect Model and Scaffold Transplantation
5:21
In Vivo Imaging of Angiogenesis within the Bone Defect
7:00
Results: 3D Scaffolds Promote Angiogenesis in Bone Defects
8:33
Conclusion
Transcription
The overall goal of this experiment is to visualize In Vivo blood vessel formation in three dimensional scaffolds in real time by multi photon microscopy. These measured are key question in bone generating field such as how to neutralize and trick
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Here, we present a protocol to visualize blood vessel formation in vivo and in real-time in 3D scaffolds by multiphoton microscopy. Angiogenesis in genetically modified scaffolds was studied in a murine calvarial critical bone defect model. More new blood vessels were detected in the treatment group than in controls.