Placing the Vestibular or the Auditory Sensory Organ in a Collagen I Gel
4:45
Viral Injections in Three-dimensional Cultures of Vestibular and Auditory Sensory Organs
6:06
Results: Use of Three-dimensional Organotypic Culture in Collagen I Gel to Study the Architecture and Viral Infections of the Vestibular and Cochlear Sensory Organs
7:40
Conclusion
Transcription
The overall goal of this procedure is to establish three-dimensional organotypic cultures of the murine vestibular and auditory sensory organs. This method can help answer the key questions in the inner ear research field, such as, what are the ro
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Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.