Results: DNA Staining with Thiazole Orange in Electrophoresis Experiments
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Conclusion
Transcription
This protocol is an easy and inexpensive alternative to using ethidium bromide for DNA detection during electrophoresis. By removing ethidium bromide, either UV light or blue light can be used for detection. Blue light is not damaging to DNA, whic
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Here, we present a protocol to use thiazole orange for the detection of DNA in gel electrophoresis experiments. The use of thiazole orange allows elimination of ethidium bromide, and fluorescence detection can be achieved with either UV or blue light.