Perivitelline Injection of Mammalian Cancer Cells into 2 dpf (Day Post Fertilization) Embryos
3:55
Live Imaging and Quantitation of the Angiogenic Response to the Zebrafish Xenograft
8:34
Results: Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
10:37
Conclusion
Transcription
This protocol describes how transgenic zebrafish larvae can be used to provide an in vivo quantitative assay of tumor xenograft vascularization. The main advantage of this technique over previous techniques is that it allows the investigator to ac
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The aim of this method is to generate an in vivo model of tumor angiogenesis by xenografting mammalian tumor cells into a zebrafish embryo that has fluorescently-labelled blood vessels. By imaging the xenograft and associated vessels, a quantitative measurement of the angiogenic response can be obtained.