Results: Extension and Relaxation of VWF and Lambda DNA Molecules due to Shear Flow
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Conclusion
Transcription
Quantifying the conformational changes in single bio-molecules under shear flow is useful for understanding the function and biophysics of macromolecules like protein and DNA. Fluorescence microscopy allows real-time in-situ visualization of singl
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We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.