Eyeball Cleaning, Retina Extraction, Transfer, and Washing
3:06
Retina Dissociation
5:20
Cell Passage to Remove Neuronal Survivors
7:12
Results: Tracking of Mller Glia Conversion into Progenitor Cells and Neuronal Identity Validation
9:24
Conclusion
Transcription
This protocol allows to study the potential and the capability of Muller glia to convert into retinal progenitor cells after treatment with specific factors such as microRNAs. The advantage of this technique is that microRNA candidates can be test
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Müller glia primary cultures obtained from mouse retinas represent a very robust and reliable tool to study the glial conversion into retinal progenitor cells after microRNA treatment. Single molecules or combinations can be tested before their subsequent application of in vivo approaches.