Preparation of Liposomes Based on the Inverted Emulsion Techniques
5:51
Microscopy Observation
8:11
Results: Microscopic Images of the Monolayer Lipid Droplet and Encapsulation of Actin Networks Inside Liposomes
9:27
Conclusion
Transcription
This measure builds a minimum model of the cell devoid of complex biochemical regulations. The technique used can generate high yield of liposomes and have high encapsulation efficiencies for cytoskeleton proteins. This measure can be applied to e
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In this manuscript, we demonstrate the experimental techniques to encapsulate the F-actin cytoskeleton into giant unilamellar lipid vesicles (also called liposomes), and the method to form a cortex-biomimicking F-actin layer at the inner leaflet of the liposome membrane.