Human induced pluripotent stem cell-derived cardiomyocytes represent a powerful tool for studying mutation-mediated changes in cardiomyocyte function and defining the effects of stressors and drug interventions. There's a need for a platform in which contractility and calcium handling of human induced pluripotent stem cell-derived cardiomyocytes can be measured in a user-friendly and reproducible manner. This method enables researchers to study contractility of human-induced pluripotent stem cell-derived cardiomyocytes by pixel correlation and measuring intracellular calcium transients simultaneously by loading the cells with calcium-sensitive fluorophore.
By using this platform, it's possible to perform paired measurements in a well-preserved temperature environment and different plate formats, and to access the data instantly. This method enables the study of cellular pathomechanisms and evaluates the effect of compounds and the functionality of human iPSC-derived cardiomyocytes, that can be helpful in the preclinical drug screening process.
