Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived CardiomyocytesRafeeh Dinani 1,2, Emmy Manders 1,2,3, Michiel Helmes 1,2,3, Lili Wang 4, Bjorn C. Knollmann 4, Diederik W. D. Kuster 1,2, Jolanda van der Velden 1,2
1Division of Physiology, Amsterdam UMC, Vrije University, 2Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, 3CytoCypher BV, 4Division of Clinical Pharmacology, Vanderbilt School of Medicine
Here, an established method is described to perform contractility and calcium measurements in human induced pluripotent stem cell-derived cardiomyocytes using an optics-based platform. This platform enables researchers to study the effect of mutations and the response to various stimuli in a rapid and reproducible manner.
