discovering-protein-interactions-characterizing-protein-function

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

Research in proteomics has exploded in recent years with advances in mass spectrometry capabilities that have led to the characterization of numerous ...

MS BIoworks LLC

High resolution autoradiography at the cellular level localized endothelin-1 binding sites to the collecting ducts in the rat renal papilla. These receptors were functionally coupled to the phosphatidyl-inositol system since endothelin-1 stimulated the accumulation of IP(1) in a concentration-dependent manner in cross chopped slices from renal papillae. The concentration-effect curves in 4 week old normotensive Wistar-Kyoto rats (WKY) lay to the right of curves from 4 week old and adult spontaneously hypertensive rats (SHR) and adult WKY (20-24 week old) animals; the EC(50) values in the 4 week old animals were 17 +/- 5 and 8 +/- 1 nM (P < 0.05, n = 5; mean +/- SE) for the normotensive and hypertensive animals, respectively. Autoradiographic studies showed that the density and distribution of binding sites for [(125)I]endothelin-1 in the kidneys did not differ between the groups; receptor densities in the renal papillae were 461 +/- 37 (fmol/mg protein) in the 4 week old WKY, and 443 +/- 27 (fmol/mg protein) in the 4 week old SHR. The plasma levels of immunoreactive endothelin-1 were also similar between groups; 4 week old SHR (39 +/- 3 pg/ml) and 4 week old WKY (36 +/- 1 pg/ml). The increased response to endothelin-1 may be related to the development of the hypertensive state in the SHR.

Richard Jones

MS BIoworks LLC

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology